SALIVARY sEV ISOLATION COMPARISON

Goal

GOAL

Compare acoustofluidics sEV/exosome isolation to widely used methods: ExoQuick-TC and Differential Ultracentrifuge Performed by commercial service provider

Problem

PROBLEM

• Ultracentrifuge introduce extensive amount of heat and takes several hours to several days for one batch
• Precipitation method tend to introduce unwanted content and impact the integrity of EV

What we do

WHAT WE DID

• Isolate saliva samples from three donors with Ultracentrifuge, Precipitation, and acoustofluidic method
• Characterize the result using TEM, NTA, and western blotting

Sample type: Saliva

Case Study

02

Results

Results

Results presented and shared at NIH ERCC19 conference hosted April, 2023,
• Acoustofluidic separation recovers 15 times higher sEV and total RNA from the same volume of input saliva sample measured by NTA and Nanodrop.

• TEM images show the acoustofluidic separation of sEV from saliva has a better shape and more intact structure.

SALIVARY sEV ISOLATION COMPARISON study

part of NIH Rigor & Reproducibility Study
The Extracellular RNA Communication Consortium (ERCC) is an NIH Common Fund program launched in 2013 to study extracellular RNA (exRNA)
https://exrna.org/ercc19/

Research collaborators:

David T. Wong, UCLA
Louise C. Laurent, UCSD
John P. Nolan, Scintillon Institute
Tony Jun Huang, Duke University

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